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@ARTICLE{Yang:837812,
      author       = {Yang, Ge and Yu, Kun and Kubicek, Jan and Labahn, Jörg},
      title        = {{E}xpression, purification, and preliminary
                      characterization of human presenilin-2},
      journal      = {Process biochemistry},
      volume       = {64},
      issn         = {1359-5113},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2017-06601},
      pages        = {63-73},
      year         = {2018},
      abstract     = {Presenilins (PS1 and PS2) exhibit similar
                      γ-secretase-dependent and −independent functions with
                      subtle variations. In this study, we established a
                      cost-effective process to overexpress and purify full-length
                      human PS2 in sufficient quantities and quality for
                      structural studies. Upon optimization, milligram quantities
                      of homogeneous trimeric hisPS2 were purified, which enabled
                      the preliminary characterization of human hisPS2 zymogen.
                      Far-UV and near-UV CD as well as fluorescence spectroscopy
                      revealed that purified hisPS2 contained the expected
                      secondary structure and was folded into a defined tertiary
                      structure. Thermal stability analysis revealed a Tm value of
                      ∼55 °C for secondary structure while cholesterol
                      significantly increased the stability. The low melting
                      temperature of ∼34 °C for the tertiary structure was able
                      to explain the purity and aggregation problems observed
                      during purification. Additionally, the occurrence of calcium
                      ions induced structural changes to different extents for
                      PS2WT and PS2-D263A/D366A was observed, which is consistent
                      with previous studies.},
      cin          = {ICS-6},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000423003200008},
      doi          = {10.1016/j.procbio.2017.09.012},
      url          = {https://juser.fz-juelich.de/record/837812},
}