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000837950 1001_ $$0P:(DE-Juel1)133466$$aUnverricht, Marcus$$b0$$eCorresponding author$$ufzj
000837950 1112_ $$aJoint Meeting of the European Radiation Research Society  and the Society for Biological Radiation Research$$cEssen$$d2017-09-17 - 2017-09-21$$gERRS and GBS$$wGermany
000837950 245__ $$aComet Assay analysis of DNA strand breaks in human cells after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125
000837950 260__ $$c2017
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000837950 520__ $$aIonizing radiation (IR) causes various types of DNA damages e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted towards the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1  is calculated based on computer simulations (Pomplun et al. 1996). In the presented work the SSB/DSB ratio induced by Iodine-125 was experimentally determined and compared to external homogenous γ-irradiation. SCL-II cells were labeled with I-125-iododeoxyuridine (I-125-UdR) and subsequently frozen for decay accumulation. Accordingly, external γ-irradiation (Cs-137 source) experiments were also performed in frozen cells. After exposure cells were thawed and used for the neutral or alkaline Comet Assay to quantify DSB or DSB & SSB, respectively. To avoid dose calculation for DNA-associated Iodine-125 the γ-H2AX assay was used in order to allow the comparison of the Comet Assay data between both investigated radiation qualities.Preliminary results show a lower SSB/DSB ratio induced by I-125-UdR when compared to external low-LET γ radiation comparable to the calculated values of Pomplun et al. (1996). This indicates that DNA-incorporated Iodine-125 induces a high-LET type DNA damage pattern.  Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK043AReference:Pomplun, E.; Terrissol, M.; Demonchy, M. (1996): Modelling of Initial Events and Chemical Behaviour of Species Induced in DNA Units by Auger Electrons from 125I, 123I and Carbon. Acta Oncologica 35(7): 857–862
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000837950 7001_ $$0P:(DE-Juel1)169961$$aHoltmann, Kathrin$$b1
000837950 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b2$$ufzj
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