Poster (After Call)

FZJ-2017-06711

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Comet Assay analysis of DNA strand breaks in human cells after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125

Unverricht, M. (Corresponding author)FZJ* ; Holtmann, K. ; Kriehuber, R.FZJ*

2017

Please use a persistent id in citations: http://hdl.handle.net/2128/16222

Abstract: Ionizing radiation (IR) causes various types of DNA damages e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted towards the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simulations (Pomplun et al. 1996). In the presented work the SSB/DSB ratio induced by Iodine-125 was experimentally determined and compared to external homogenous γ-irradiation. SCL-II cells were labeled with I-125-iododeoxyuridine (I-125-UdR) and subsequently frozen for decay accumulation. Accordingly, external γ-irradiation (Cs-137 source) experiments were also performed in frozen cells. After exposure cells were thawed and used for the neutral or alkaline Comet Assay to quantify DSB or DSB & SSB, respectively. To avoid dose calculation for DNA-associated Iodine-125 the γ-H2AX assay was used in order to allow the comparison of the Comet Assay data between both investigated radiation qualities.Preliminary results show a lower SSB/DSB ratio induced by I-125-UdR when compared to external low-LET γ radiation comparable to the calculated values of Pomplun et al. (1996). This indicates that DNA-incorporated Iodine-125 induces a high-LET type DNA damage pattern. Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK043AReference:Pomplun, E.; Terrissol, M.; Demonchy, M. (1996): Modelling of Initial Events and Chemical Behaviour of Species Induced in DNA Units by Auger Electrons from 125I, 123I and Carbon. Acta Oncologica 35(7): 857–862

Keyword(s): Biology (2nd)

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Contributing Institute(s):

1. Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie (S-US)

Research Program(s):

1. 899 - ohne Topic (POF3-899) (POF3-899)

Appears in the scientific report 2017

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Database coverage:

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