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@INPROCEEDINGS{Unverricht:837950,
      author       = {Unverricht, Marcus and Holtmann, Kathrin and Kriehuber,
                      Ralf},
      title        = {{C}omet {A}ssay analysis of {DNA} strand breaks in human
                      cells after exposure to the {DNA}-incorporated {A}uger
                      {E}lectron {E}mitter {I}odine-125},
      reportid     = {FZJ-2017-06711},
      year         = {2017},
      abstract     = {Ionizing radiation (IR) causes various types of DNA damages
                      e.g. single strand breaks (SSB) and double strand breaks
                      (DSB), whereby the SSB/DSB ratio is shifted towards the DSB
                      with increasing LET. For the DNA-incorporated Auger electron
                      emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated
                      based on computer simulations (Pomplun et al. 1996). In the
                      presented work the SSB/DSB ratio induced by Iodine-125 was
                      experimentally determined and compared to external
                      homogenous γ-irradiation. SCL-II cells were labeled with
                      I-125-iododeoxyuridine (I-125-UdR) and subsequently frozen
                      for decay accumulation. Accordingly, external γ-irradiation
                      (Cs-137 source) experiments were also performed in frozen
                      cells. After exposure cells were thawed and used for the
                      neutral or alkaline Comet Assay to quantify DSB or DSB $\&$
                      SSB, respectively. To avoid dose calculation for
                      DNA-associated Iodine-125 the γ-H2AX assay was used in
                      order to allow the comparison of the Comet Assay data
                      between both investigated radiation qualities.Preliminary
                      results show a lower SSB/DSB ratio induced by I-125-UdR when
                      compared to external low-LET γ radiation comparable to the
                      calculated values of Pomplun et al. (1996). This indicates
                      that DNA-incorporated Iodine-125 induces a high-LET type DNA
                      damage pattern. Funded by Bundesministerium für Bildung und
                      Forschung (BMBF), Grant 02NUK043AReference:Pomplun, E.;
                      Terrissol, M.; Demonchy, M. (1996): Modelling of Initial
                      Events and Chemical Behaviour of Species Induced in DNA
                      Units by Auger Electrons from 125I, 123I and Carbon. Acta
                      Oncologica 35(7): 857–862},
      month         = {Sep},
      date          = {2017-09-17},
      organization  = {Joint Meeting of the European
                       Radiation Research Society and the
                       Society for Biological Radiation
                       Research, Essen (Germany), 17 Sep 2017
                       - 21 Sep 2017},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/837950},
}