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| Home > Publications database > Comet Assay analysis of DNA strand breaks in human cells after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125 > print |
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| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a Joint Meeting of the European Radiation Research Society and the Society for Biological Radiation Research |g ERRS and GBS |c Essen |d 2017-09-17 - 2017-09-21 |w Germany |
| 245 | _ | _ | |a Comet Assay analysis of DNA strand breaks in human cells after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125 |
| 260 | _ | _ | |c 2017 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
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| 520 | _ | _ | |a Ionizing radiation (IR) causes various types of DNA damages e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted towards the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simulations (Pomplun et al. 1996). In the presented work the SSB/DSB ratio induced by Iodine-125 was experimentally determined and compared to external homogenous γ-irradiation. SCL-II cells were labeled with I-125-iododeoxyuridine (I-125-UdR) and subsequently frozen for decay accumulation. Accordingly, external γ-irradiation (Cs-137 source) experiments were also performed in frozen cells. After exposure cells were thawed and used for the neutral or alkaline Comet Assay to quantify DSB or DSB & SSB, respectively. To avoid dose calculation for DNA-associated Iodine-125 the γ-H2AX assay was used in order to allow the comparison of the Comet Assay data between both investigated radiation qualities.Preliminary results show a lower SSB/DSB ratio induced by I-125-UdR when compared to external low-LET γ radiation comparable to the calculated values of Pomplun et al. (1996). This indicates that DNA-incorporated Iodine-125 induces a high-LET type DNA damage pattern. Funded by Bundesministerium für Bildung und Forschung (BMBF), Grant 02NUK043AReference:Pomplun, E.; Terrissol, M.; Demonchy, M. (1996): Modelling of Initial Events and Chemical Behaviour of Species Induced in DNA Units by Auger Electrons from 125I, 123I and Carbon. Acta Oncologica 35(7): 857–862 |
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