TY  - JOUR
AU  - Vitiello, Giuseppe
AU  - Musumeci, Domenica
AU  - Koutsioumpas, Alexandros
AU  - Paduano, Luigi
AU  - Montesarchio, Daniela
AU  - D'Errico, Gerardino
TI  - Ionophores at work: Exploring the interaction of guanosine-based amphiphiles with phospholipid membranes
JO  - Biochimica et biophysica acta / Biomembranes
VL  - 1859
IS  - 12
SN  - 0005-2736
CY  - Amsterdam
PB  - Elsevier
M1  - FZJ-2017-06761
SP  - 2392 - 2401
PY  - 2017
AB  - An amphiphilic derivative of guanosine, carrying a myristoyl group at the 5′-position and two methoxy(triethylene glycol) appendages at the 2′ and 3′-positions (1), endowed with high ionophoric activity, has been here studied in its interaction mode with a model lipid membrane along with its 5′-spin-labelled analogue 2, bearing the 5-doxyl-stearic in lieu of the myristic residue. Electron spin resonance spectra, carried out on the spin-labelled nucleolipid 2 in mixture with a DOPC/DOPG phospholipid bilayer, on one side, and on spin-labelled lipids mixed with 1, on the other, integrated with dynamic light scattering and neutron reflectivity measurements, allowed getting an in-depth picture of the effect of the ionophores on membrane structure, relevant to clarify the ion transport mechanism through lipid bilayers. Particularly, dehydration of lipid headgroups and lowering of both the local polarity and acyl chains order across the bilayer, due to the insertion of the oligo(ethylene glycol) chains in the bilayer hydrophobic core, have been found to be the main effects of the amphiphilic guanosines interaction with the membrane. These results furnish directions to rationally implement future ionophores design.
LB  - PUB:(DE-HGF)16
C6  - pmid:28890186
UR  - <Go to ISI:>//WOS:000415770900012
DO  - DOI:10.1016/j.bbamem.2017.09.007
UR  - https://juser.fz-juelich.de/record/838018
ER  -