Journal Article

FZJ-2017-06761

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ionophores at work: Exploring the interaction of guanosine-based amphiphiles with phospholipid membranes

Vitiello, G. ; Musumeci, D. ; Koutsioumpas, A.FZJ* ; Paduano, L. ; Montesarchio, D. (Corresponding author) ; D'Errico, G. (Corresponding author)

2017
Elsevier Amsterdam

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Please use a persistent id in citations: http://hdl.handle.net/2128/15929  doi:10.1016/j.bbamem.2017.09.007

Abstract: An amphiphilic derivative of guanosine, carrying a myristoyl group at the 5′-position and two methoxy(triethylene glycol) appendages at the 2′ and 3′-positions (1), endowed with high ionophoric activity, has been here studied in its interaction mode with a model lipid membrane along with its 5′-spin-labelled analogue 2, bearing the 5-doxyl-stearic in lieu of the myristic residue. Electron spin resonance spectra, carried out on the spin-labelled nucleolipid 2 in mixture with a DOPC/DOPG phospholipid bilayer, on one side, and on spin-labelled lipids mixed with 1, on the other, integrated with dynamic light scattering and neutron reflectivity measurements, allowed getting an in-depth picture of the effect of the ionophores on membrane structure, relevant to clarify the ion transport mechanism through lipid bilayers. Particularly, dehydration of lipid headgroups and lowering of both the local polarity and acyl chains order across the bilayer, due to the insertion of the oligo(ethylene glycol) chains in the bilayer hydrophobic core, have been found to be the main effects of the amphiphilic guanosines interaction with the membrane. These results furnish directions to rationally implement future ionophores design.

Keyword(s): Health and Life (1st) ; Soft Condensed Matter (2nd) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)

Research Program(s):

1. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
2. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)

Experiment(s):

1. MARIA: Magnetic reflectometer with high incident angle (NL5N)

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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