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@INPROCEEDINGS{Unverricht:841298,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{F}lowcytometrical analysis of radiation-induced
                      γ-{H}2{AX} foci},
      reportid     = {FZJ-2017-08388},
      year         = {2009},
      abstract     = {PURPOSE: Phosphorylation of histone H2AX (γ-H2AX) occurs
                      at sites flanking DNA double-strand breaks (DSBs) and can
                      provide a measure of the number of DSBs within a cell
                      (γ-H2AX-assay). We investigated whether the mean intensity
                      and the mean number of radiation-induced γ-H2AX foci vary
                      as a function of radiation quality and dose.MATERIALS AND
                      METHODS: Jurkat cells were irradiated with different doses
                      of either low linear energy transfer (LET) 137Cs γ-rays or
                      high LET 241Am α-particles. The γ-H2AX foci were detected
                      using immunocytochemistry (primary antibody
                      Anti-phospho-Histone H2AX(Ser139) mouse IgG, secondary
                      antibody FITC goat anti-mouse IgG) and quantified by
                      counting the number of γ-H2AX foci employing fluorescence
                      microscopy and by measuring the mean signal intensity in
                      single cell nuclei by flow cytometry. RESULTS: The mean
                      number of γ-H2AX foci is increased in a dose dependent
                      manner for both radiation qualities and are broadly similar
                      at identical absorbed radiation dose for both investigated
                      radiation qualities. The mean γ-H2AX signal intensity of
                      single nuclei is increased after alpha-irradiation when
                      compared to γ-irradiation at the same absorbed radiation
                      dose. CONCLUSIONS: α-particle induced γ-H2AX foci show
                      higher signal intensities compared to γ-ray-induced γ-H2AX
                      foci. The mean intensity of radiation-induced γ-H2AX foci
                      is dependent on radiation quality in Jurkat cells.},
      month         = {Oct},
      date          = {2009-10-14},
      organization  = {19th Annual Conference of the German
                       Society for Cytometry, Leipzig
                       (Germany), 14 Oct 2009 - 16 Oct 2009},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841298},
}