Poster (After Call)

FZJ-2017-08388

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Flowcytometrical analysis of radiation-induced γ-H2AX foci

Unverricht, M. (Corresponding author)FZJ* ; Giesen, U. ; Kriehuber, R.FZJ*

2009

Please use a persistent id in citations: http://hdl.handle.net/2128/16244

Abstract: PURPOSE: Phosphorylation of histone H2AX (γ-H2AX) occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell (γ-H2AX-assay). We investigated whether the mean intensity and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry (primary antibody Anti-phospho-Histone H2AX(Ser139) mouse IgG, secondary antibody FITC goat anti-mouse IgG) and quantified by counting the number of γ-H2AX foci employing fluorescence microscopy and by measuring the mean signal intensity in single cell nuclei by flow cytometry. RESULTS: The mean number of γ-H2AX foci is increased in a dose dependent manner for both radiation qualities and are broadly similar at identical absorbed radiation dose for both investigated radiation qualities. The mean γ-H2AX signal intensity of single nuclei is increased after alpha-irradiation when compared to γ-irradiation at the same absorbed radiation dose. CONCLUSIONS: α-particle induced γ-H2AX foci show higher signal intensities compared to γ-ray-induced γ-H2AX foci. The mean intensity of radiation-induced γ-H2AX foci is dependent on radiation quality in Jurkat cells.

Keyword(s): Biology (2nd)

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Contributing Institute(s):

1. Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie (S-US)

Research Program(s):

1. 899 - ohne Topic (POF3-899) (POF3-899)

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Database coverage:

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