Hauptseite > Publikationsdatenbank > Flowcytometrical analysis of radiation-induced γ-H2AX foci > print

001     841298
005     20210129232009.0
024 7 _ |a 2128/16244
|2 Handle
037 _ _ |a FZJ-2017-08388
041 _ _ |a English
100 1 _ |a Unverricht, Marcus
|0 P:(DE-Juel1)133466
|b 0
|e Corresponding author
|u fzj
111 2 _ |a 19th Annual Conference of the German Society for Cytometry
|g DGfZ
|c Leipzig
|d 2009-10-14 - 2009-10-16
|w Germany
245 _ _ |a Flowcytometrical analysis of radiation-induced γ-H2AX foci
260 _ _ |c 2009
336 7 _ |a Conference Paper
|0 33
|2 EndNote
336 7 _ |a INPROCEEDINGS
|2 BibTeX
336 7 _ |a conferenceObject
|2 DRIVER
336 7 _ |a CONFERENCE_POSTER
|2 ORCID
336 7 _ |a Output Types/Conference Poster
|2 DataCite
336 7 _ |a Poster
|b poster
|m poster
|0 PUB:(DE-HGF)24
|s 1513263719_28639
|2 PUB:(DE-HGF)
|x After Call
520 _ _ |a PURPOSE: Phosphorylation of histone H2AX (γ-H2AX) occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell (γ-H2AX-assay). We investigated whether the mean intensity and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry (primary antibody Anti-phospho-Histone H2AX(Ser139) mouse IgG, secondary antibody FITC goat anti-mouse IgG) and quantified by counting the number of γ-H2AX foci employing fluorescence microscopy and by measuring the mean signal intensity in single cell nuclei by flow cytometry. RESULTS: The mean number of γ-H2AX foci is increased in a dose dependent manner for both radiation qualities and are broadly similar at identical absorbed radiation dose for both investigated radiation qualities. The mean γ-H2AX signal intensity of single nuclei is increased after alpha-irradiation when compared to γ-irradiation at the same absorbed radiation dose. CONCLUSIONS: α-particle induced γ-H2AX foci show higher signal intensities compared to γ-ray-induced γ-H2AX foci. The mean intensity of radiation-induced γ-H2AX foci is dependent on radiation quality in Jurkat cells.
536 _ _ |a 899 - ohne Topic (POF3-899)
|0 G:(DE-HGF)POF3-899
|c POF3-899
|f POF III
|x 0
650 2 7 |a Biology
|0 V:(DE-MLZ)SciArea-160
|2 V:(DE-HGF)
|x 0
700 1 _ |a Giesen, Ulrich
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Kriehuber, Ralf
|0 P:(DE-Juel1)133469
|b 2
|u fzj
856 4 _ |y OpenAccess
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.pdf
856 4 _ |y OpenAccess
|x icon
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.gif?subformat=icon
856 4 _ |y OpenAccess
|x icon-1440
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.jpg?subformat=icon-1440
856 4 _ |y OpenAccess
|x icon-180
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.jpg?subformat=icon-180
856 4 _ |y OpenAccess
|x icon-640
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.jpg?subformat=icon-640
856 4 _ |y OpenAccess
|x pdfa
|u https://juser.fz-juelich.de/record/841298/files/FZJ-2014-03890%20Poster.pdf?subformat=pdfa
909 C O |o oai:juser.fz-juelich.de:841298
|p openaire
|p open_access
|p VDB
|p driver
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 0
|6 P:(DE-Juel1)133466
910 1 _ |a Forschungszentrum Jülich
|0 I:(DE-588b)5008462-8
|k FZJ
|b 2
|6 P:(DE-Juel1)133469
913 1 _ |a DE-HGF
|b Programmungebundene Forschung
|l ohne Programm
|1 G:(DE-HGF)POF3-890
|0 G:(DE-HGF)POF3-899
|2 G:(DE-HGF)POF3-800
|v ohne Topic
|x 0
|4 G:(DE-HGF)POF
|3 G:(DE-HGF)POF3
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
920 _ _ |l yes
920 1 _ |0 I:(DE-Juel1)S-US-20090406
|k S-US
|l Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie
|x 0
980 _ _ |a poster
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-Juel1)S-US-20090406
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21