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@ARTICLE{Unverricht:845520,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{C}omparative gene expression analysis after exposure to
                      $^{123}${I}-iododeoxyuridine, γ- and
                      α-radiation—potential biomarkers for the discrimination
                      of radiation qualities},
      journal      = {Journal of radiation research},
      volume       = {59},
      number       = {4},
      issn         = {0449-3060},
      address      = {Kawaguchi},
      publisher    = {J-STAGE},
      reportid     = {FZJ-2018-02754},
      pages        = {411–429},
      year         = {2018},
      abstract     = {Gene expression analysis was carried out in Jurkat cells in
                      order to identify candidate genes showing significant gene
                      expression alterations allowing robust discrimination of the
                      Auger emitter $^{123}$I, incorporated into the DNA as
                      $^{123}$I-iododeoxyuridine ($^{123}$IUdR), from α- and
                      γ-radiation. The γ-H2AX foci assay was used to determine
                      equi-effect doses or activity, and gene expression analysis
                      was carried out at similar levels of foci induction.
                      Comparative gene expression analysis was performed employing
                      whole human genome DNA microarrays. Candidate genes had to
                      show significant expression changes and no altered gene
                      regulation or opposite regulation after exposure to the
                      radiation quality to be compared. The gene expression of all
                      candidate genes was validated by quantitative real-time PCR.
                      The functional categorization of significantly deregulated
                      genes revealed that chromatin organization and apoptosis
                      were generally affected. After exposure to $^{123}$IUdR,
                      α-particles and γ-rays, at equi-effect doses/activity,
                      155, 316 and 982 genes were exclusively regulated,
                      respectively. Applying the stringent requirements for
                      candidate genes, four (PPP1R14C, TNFAIP8L1, DNAJC1 and
                      PRTFDC1), one (KLF10) and one (TNFAIP8L1) gene(s) were
                      identified, respectively allowing reliable discrimination
                      between γ- and $^{123}$IUdR exposure, γ- and α-radiation,
                      and α- and 123IUdR exposure, respectively. The Auger
                      emitter $^{123}$I induced specific gene expression patterns
                      in Jurkat cells when compared with γ- and α-irradiation,
                      suggesting a unique cellular response after $^{123}$IUdR
                      exposure. Gene expression analysis might be an effective
                      tool for identifying biomarkers for discriminating different
                      radiation qualities and, furthermore, might help to explain
                      the varying biological effectiveness at the mechanistic
                      level.},
      cin          = {S-US / ZEA-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)S-US-20090406 / I:(DE-Juel1)ZEA-1-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29800458},
      UT           = {WOS:000439732900004},
      doi          = {10.1093/jrr/rry038},
      url          = {https://juser.fz-juelich.de/record/845520},
}