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@ARTICLE{Prerostova:846019,
author = {Prerostova, Sylva and Dobrev, Petre I. and Gaudinova, Alena
and Knirsch, Vojtech and Körber, Niklas and Pieruschka,
Roland and Fiorani, Fabio and Brzobohatý, Břetislav and
černý, Martin and Spichal, Lukas and Humplik, Jan and
Vanek, Tomas and Schurr, Ulrich and Vankova, Radomira},
title = {{C}ytokinins: {T}heir {I}mpact on {M}olecular and {G}rowth
{R}esponses to {D}rought {S}tress and {R}ecovery in
{A}rabidopsis},
journal = {Frontiers in Functional Plant Ecology},
volume = {9},
issn = {1664-462X},
address = {Lausanne},
publisher = {Frontiers Media88991},
reportid = {FZJ-2018-03189},
pages = {655},
year = {2018},
abstract = {Our phenotyping and hormonal study has characterized the
role of cytokinins (CK) in the drought and recovery
responses of Arabidopsis thaliana. CK down-regulation was
achieved by overexpression of the gene for CK deactivating
enzyme cytokinin oxidase/dehydrogenase (CKX): constitutive
(35S:CKX) or at the stress onset using a
dexamethasone-inducible pOp/LhGR promoter (DEX:CKX). The
35S:CKX plants exhibited slow ontogenesis and higher
expression levels of stress-associated genes, e.g., AtP5CS1,
already at well-watered conditions. CK down-regulation
resulted during drought in higher stress tolerance
(indicated by relatively low up-regulation of the expression
of drought stress marker gene AtRD29B) accompanied with
lower leaf water loss. Nevertheless, these plants exhibited
slow and delayed recovery after re-watering. CK levels were
increased at the stress onset by stimulation of the
expression of CK biosynthetic gene isopentenyl transferase
(ipt) (DEX:IPT) or by application of exogenous CK
meta-topolin. After water withdrawal, long-term CK elevation
resulted in higher water loss in comparison with CKX
transformants as well as with plants overexpressing ipt
driven by senescence-inducible SAG12 promoter (SAG:IPT),
which gradually enhanced CKs during the stress progression.
In all cases, CK up-regulation resulted in fast and more
vigorous recovery. All drought-stressed plants exhibited
growth suppression associated with elevation of abscisic
acid and decrease of auxins and active CKs (with the
exception of SAG:IPT plants). Apart from the ipt
overexpressers, also increase of jasmonic and salicylic acid
was found.},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582) / EPPN - European Plant
Phenotyping Network (284443)},
pid = {G:(DE-HGF)POF3-582 / G:(EU-Grant)284443},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000432651900001},
pubmed = {pmid:29872444},
doi = {10.3389/fpls.2018.00655},
url = {https://juser.fz-juelich.de/record/846019},
}