Journal Article

FZJ-2018-03243

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Relevance of Internal Friction and Structural Constraints for the Dynamics of Denatured Bovine Serum Albumin

Ameseder, F.FZJ* ; Radulescu, A.MLZ*FZJ* ; Holderer, O.MLZ*FZJ* ; Falus, P. ; Richter, D.FZJ* ; Stadler, A. M. (Corresponding author)FZJ*

2018
ACS Washington, DC

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Please use a persistent id in citations: doi:10.1021/acs.jpclett.8b00825

Abstract: A general property of disordered proteins is their structural expansion that results in a high molecular flexibility. The structure and dynamics of bovine serum albumin (BSA) denatured by guanidinium hydrochloride (GndCl) were investigated using small-angle neutron scattering (SANS) and neutron spin–echo spectroscopy (NSE). SANS experiments demonstrated the relevance of intrachain interactions for structural expansion. Using NSE experiments, we observed a high internal flexibility of denatured BSA in addition to center-of-mass diffusion detected by dynamic light scattering. Internal motions measured by NSE were described using concepts based on polymer theory. The contribution of residue-solvent friction was accounted for using the Zimm model including internal friction (ZIF). Disulfide bonds forming loops of amino acids of the peptide backbone have a major impact on internal dynamics that can be interpreted with a reduced set of Zimm modes.

Keyword(s): Polymers, Soft Nano Particles and Proteins (1st) ; Soft Condensed Matter (2nd) ; Biology (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II)
2. Neutronenstreuung (Neutronenstreuung ; JCNS-1)
3. Streumethoden (JCNS-2)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)
3. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)

Experiment(s):

1. KWS-2: Small angle scattering diffractometer (NL3ao)
2. J-NSE: Neutron spin-echo spectrometer (NL2ao)

Appears in the scientific report 2018

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Database coverage:
; Current Contents - Physical, Chemical and Earth Sciences ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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