%0 Journal Article
%A Endepols, Heike
%A Mottaghy, Felix M.
%A Simsekyilmaz, Sakine
%A Bucerius, Jan
%A Vogt, Felix
%A Winz, Oliver
%A Richarz, Raphael
%A Krapf, Philipp
%A Neumaier, Bernd
%A Zlatopolskiy, Boris D.
%A Morgenroth, Agnieszka
%T In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
%J Scientific reports
%V 8
%N 1
%@ 2045-2322
%C London
%I Nature Publishing Group
%M FZJ-2018-03667
%P 7411
%D 2018
%X The short- and long-term success of intravascular stents depends on a proper re-endothelialisation afterthe intervention-induced endothelial denudation. The aim of this study was to evaluate the potentialof in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specificmembrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawleyrats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emissiontomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracersolution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specificantibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis.[18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with anipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII inendothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verifiedthe site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we providethe first non-invasive in vivo delineation of re-endothelialisation after angioplasty.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:29743623
%U <Go to ISI:>//WOS:000431737300097
%R 10.1038/s41598-018-25863-1
%U https://juser.fz-juelich.de/record/848432