Journal Article

FZJ-2018-03667

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model

Endepols, H. ; Mottaghy, F. M. (Corresponding author)FZJ* ; Simsekyilmaz, S. ; Bucerius, J. ; Vogt, F. ; Winz, O. ; Richarz, R. ; Krapf, P.FZJ* ; Neumaier, B.FZJ* ; Zlatopolskiy, B. D. ; Morgenroth, A.

2018
Nature Publishing Group London

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Please use a persistent id in citations: http://hdl.handle.net/2128/19180  doi:10.1038/s41598-018-25863-1

Abstract: The short- and long-term success of intravascular stents depends on a proper re-endothelialisation afterthe intervention-induced endothelial denudation. The aim of this study was to evaluate the potentialof in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specificmembrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawleyrats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emissiontomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracersolution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specificantibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis.[18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with anipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII inendothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verifiedthe site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we providethe first non-invasive in vivo delineation of re-endothelialisation after angioplasty.

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Contributing Institute(s):

1. Nuklearchemie (INM-5)

Research Program(s):

1. 572 - (Dys-)function and Plasticity (POF3-572) (POF3-572)

Appears in the scientific report 2018

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