TY  - JOUR
AU  - Endepols, Heike
AU  - Mottaghy, Felix M.
AU  - Simsekyilmaz, Sakine
AU  - Bucerius, Jan
AU  - Vogt, Felix
AU  - Winz, Oliver
AU  - Richarz, Raphael
AU  - Krapf, Philipp
AU  - Neumaier, Bernd
AU  - Zlatopolskiy, Boris D.
AU  - Morgenroth, Agnieszka
TI  - In vivo Molecular Imaging of Glutamate Carboxypeptidase II Expression in Re-endothelialisation after Percutaneous Balloon Denudation in a Rat Model
JO  - Scientific reports
VL  - 8
IS  - 1
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2018-03667
SP  - 7411
PY  - 2018
AB  - The short- and long-term success of intravascular stents depends on a proper re-endothelialisation afterthe intervention-induced endothelial denudation. The aim of this study was to evaluate the potentialof in vivo molecular imaging of glutamate carboxypeptidase II (GCPII; identical with prostate-specificmembrane antigen PSMA) expression as a marker of re-endothelialisation. Fifteen Sprague Dawleyrats underwent unilateral balloon angioplasty of the common carotid artery (CCA). Positron emissiontomography (PET) using the GCPII-targeting tracer [18F]DCFPyL was performed after 5–21 days (scan 60–120 min post injection). In two animals, the GCPII inhibitor PMPA (23 mg/kg BW) was added to the tracersolution. After PET, both CCAs were removed, dissected, and immunostained with the GCPII specificantibody YPSMA-1. Difference of GCPII expression between both CCAs was established by PCR analysis.[18F]DCFPyL uptake was significantly higher in the ipsilateral compared to the contralateral CCA with anipsi-/contralateral ratio of 1.67 ± 0.39. PMPA blocked tracer binding. The selective expression of GCPII inendothelial cells of the treated CCA was confirmed by immunohistological staining. PCR analysis verifiedthe site-specific GCPII expression. By using a molecular imaging marker of GCPII expression, we providethe first non-invasive in vivo delineation of re-endothelialisation after angioplasty.
LB  - PUB:(DE-HGF)16
C6  - pmid:29743623
UR  - <Go to ISI:>//WOS:000431737300097
DO  - DOI:10.1038/s41598-018-25863-1
UR  - https://juser.fz-juelich.de/record/848432
ER  -