%0 Journal Article
%A Sadoine, Mayuri
%A Cerminara, Michele
%A Fitter, Jörg
%A Katranidis, Alexandros
%T Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies
%J Bio-protocol
%V 8
%N 12
%@ 2331-8325
%C Sunnyvale, CA
%I bio-protocol.org
%M FZJ-2018-03811
%P 1
%D 2018
%X Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination. 
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000457962400003
%R 10.21769/BioProtoc.2881
%U https://juser.fz-juelich.de/record/849675