Journal Article

FZJ-2018-03811

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies

Sadoine, M. ; Cerminara, M. ; Fitter, J. (Corresponding author)FZJ* ; Katranidis, A. (Corresponding author)FZJ*

2018
bio-protocol.org Sunnyvale, CA

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Please use a persistent id in citations: doi:10.21769/BioProtoc.2881

Abstract: Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination.

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Contributing Institute(s):

1. Molekulare Biophysik (ICS-5)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2018

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Database coverage:

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