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000849675 0247_ $$2doi$$a10.21769/BioProtoc.2881
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000849675 041__ $$aEnglish
000849675 082__ $$a570
000849675 1001_ $$0P:(DE-HGF)0$$aSadoine, Mayuri$$b0
000849675 245__ $$aPreparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies
000849675 260__ $$aSunnyvale, CA$$bbio-protocol.org$$c2018
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000849675 520__ $$aSingle-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination. 
000849675 536__ $$0G:(DE-HGF)POF3-551$$a551 - Functional Macromolecules and Complexes (POF3-551)$$cPOF3-551$$fPOF III$$x0
000849675 588__ $$aDataset connected to CrossRef
000849675 7001_ $$0P:(DE-HGF)0$$aCerminara, Michele$$b1
000849675 7001_ $$0P:(DE-Juel1)131961$$aFitter, Jörg$$b2$$eCorresponding author
000849675 7001_ $$0P:(DE-Juel1)131971$$aKatranidis, Alexandros$$b3$$eCorresponding author
000849675 773__ $$0PERI:(DE-600)2833269-6$$a10.21769/BioProtoc.2881$$gVol. 8, no. 12$$n12$$p1$$tBio-protocol$$v8$$x2331-8325$$y2018
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000849675 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131961$$aForschungszentrum Jülich$$b2$$kFZJ
000849675 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131971$$aForschungszentrum Jülich$$b3$$kFZJ
000849675 9131_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0
000849675 9141_ $$y2018
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000849675 9201_ $$0I:(DE-Juel1)ICS-5-20110106$$kICS-5$$lMolekulare Biophysik$$x0
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