TY  - JOUR
AU  - Sadoine, Mayuri
AU  - Cerminara, Michele
AU  - Fitter, Jörg
AU  - Katranidis, Alexandros
TI  - Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies
JO  - Bio-protocol
VL  - 8
IS  - 12
SN  - 2331-8325
CY  - Sunnyvale, CA
PB  - bio-protocol.org
M1  - FZJ-2018-03811
SP  - 1
PY  - 2018
AB  - Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination. 
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000457962400003
DO  - DOI:10.21769/BioProtoc.2881
UR  - https://juser.fz-juelich.de/record/849675
ER  -