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| Hauptseite > Publikationsdatenbank > Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies > print |
| Hauptseite > Publikationsdatenbank > Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies > print |
| 001 | 849675 | ||
| 005 | 20240610120757.0 | ||
| 024 | 7 | _ | |a 10.21769/BioProtoc.2881 |2 doi |
| 024 | 7 | _ | |a WOS:000457962400003 |2 WOS |
| 037 | _ | _ | |a FZJ-2018-03811 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Sadoine, Mayuri |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies |
| 260 | _ | _ | |a Sunnyvale, CA |c 2018 |b bio-protocol.org |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1530183240_14341 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor fluorophores. Here, we present a detailed protocol for preparing double-labeled proteins for smFRET studies. The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter‐dye distance determination. |
| 536 | _ | _ | |a 551 - Functional Macromolecules and Complexes (POF3-551) |0 G:(DE-HGF)POF3-551 |c POF3-551 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Cerminara, Michele |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Fitter, Jörg |0 P:(DE-Juel1)131961 |b 2 |e Corresponding author |
| 700 | 1 | _ | |a Katranidis, Alexandros |0 P:(DE-Juel1)131971 |b 3 |e Corresponding author |
| 773 | _ | _ | |a 10.21769/BioProtoc.2881 |g Vol. 8, no. 12 |0 PERI:(DE-600)2833269-6 |n 12 |p 1 |t Bio-protocol |v 8 |y 2018 |x 2331-8325 |
| 909 | C | O | |o oai:juser.fz-juelich.de:849675 |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)131961 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)131971 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-551 |2 G:(DE-HGF)POF3-500 |v Functional Macromolecules and Complexes |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2018 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)ICS-5-20110106 |k ICS-5 |l Molekulare Biophysik |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-5-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-6-20200312 |
| 981 | _ | _ | |a I:(DE-Juel1)ER-C-3-20170113 |
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