TY  - JOUR
AU  - Endres, Stephan
AU  - Wingen, Marcus
AU  - Torra, Joaquim
AU  - Ruiz-González, Rubén
AU  - Polen, Tino
AU  - Bosio, Gabriela
AU  - Bitzenhofer, Nora Lisa
AU  - Hilgers, Fabienne
AU  - Gensch, Thomas
AU  - Nonell, Santi
AU  - Jaeger, Karl-Erich
AU  - Drepper, Thomas
TI  - An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria
JO  - Scientific reports
VL  - 8
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - FZJ-2018-05728
SP  - 15021
PY  - 2018
AB  - Flavin-binding fluorescent proteins (FPs) are genetically encoded in vivo reporters, which are derived from microbial and plant LOV photoreceptors. In this study, we comparatively analyzed ROS formation and light-driven antimicrobial efficacy of eleven LOV-based FPs. In particular, we determined singlet oxygen (1O2) quantum yields and superoxide photosensitization activities via spectroscopic assays and performed cell toxicity experiments in E. coli. Besides miniSOG and SOPP, which have been engineered to generate 1O2, all of the other tested flavoproteins were able to produce singlet oxygen and/or hydrogen peroxide but exhibited remarkable differences in ROS selectivity and yield. Accordingly, most LOV-FPs are potent photosensitizers, which can be used for light-controlled killing of bacteria. Furthermore, the two variants Pp2FbFP and DsFbFP M49I, exhibiting preferential photosensitization of singlet oxygen or singlet oxygen and superoxide, respectively, were shown to be new tools for studying specific ROS-induced cell signaling processes. The tested LOV-FPs thus further expand the toolbox of optogenetic sensitizers usable for a broad spectrum of microbiological and biomedical applications.
LB  - PUB:(DE-HGF)16
C6  - pmid:30301917
UR  - <Go to ISI:>//WOS:000446802800027
DO  - DOI:10.1038/s41598-018-33291-4
UR  - https://juser.fz-juelich.de/record/856064
ER  -