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@ARTICLE{Endres:856064,
      author       = {Endres, Stephan and Wingen, Marcus and Torra, Joaquim and
                      Ruiz-González, Rubén and Polen, Tino and Bosio, Gabriela
                      and Bitzenhofer, Nora Lisa and Hilgers, Fabienne and Gensch,
                      Thomas and Nonell, Santi and Jaeger, Karl-Erich and Drepper,
                      Thomas},
      title        = {{A}n optogenetic toolbox of {LOV}-based photosensitizers
                      for light-driven killing of bacteria},
      journal      = {Scientific reports},
      volume       = {8},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {FZJ-2018-05728},
      pages        = {15021},
      year         = {2018},
      abstract     = {Flavin-binding fluorescent proteins (FPs) are genetically
                      encoded in vivo reporters, which are derived from microbial
                      and plant LOV photoreceptors. In this study, we
                      comparatively analyzed ROS formation and light-driven
                      antimicrobial efficacy of eleven LOV-based FPs. In
                      particular, we determined singlet oxygen (1O2) quantum
                      yields and superoxide photosensitization activities via
                      spectroscopic assays and performed cell toxicity experiments
                      in E. coli. Besides miniSOG and SOPP, which have been
                      engineered to generate 1O2, all of the other tested
                      flavoproteins were able to produce singlet oxygen and/or
                      hydrogen peroxide but exhibited remarkable differences in
                      ROS selectivity and yield. Accordingly, most LOV-FPs are
                      potent photosensitizers, which can be used for
                      light-controlled killing of bacteria. Furthermore, the two
                      variants Pp2FbFP and DsFbFP M49I, exhibiting preferential
                      photosensitization of singlet oxygen or singlet oxygen and
                      superoxide, respectively, were shown to be new tools for
                      studying specific ROS-induced cell signaling processes. The
                      tested LOV-FPs thus further expand the toolbox of
                      optogenetic sensitizers usable for a broad spectrum of
                      microbiological and biomedical applications.},
      cin          = {ICS-4 / IBG-1 / IMET},
      ddc          = {600},
      cid          = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)IBG-1-20101118 /
                      I:(DE-Juel1)IMET-20090612},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30301917},
      UT           = {WOS:000446802800027},
      doi          = {10.1038/s41598-018-33291-4},
      url          = {https://juser.fz-juelich.de/record/856064},
}