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@ARTICLE{Endres:856064,
author = {Endres, Stephan and Wingen, Marcus and Torra, Joaquim and
Ruiz-González, Rubén and Polen, Tino and Bosio, Gabriela
and Bitzenhofer, Nora Lisa and Hilgers, Fabienne and Gensch,
Thomas and Nonell, Santi and Jaeger, Karl-Erich and Drepper,
Thomas},
title = {{A}n optogenetic toolbox of {LOV}-based photosensitizers
for light-driven killing of bacteria},
journal = {Scientific reports},
volume = {8},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Macmillan Publishers Limited, part of Springer Nature},
reportid = {FZJ-2018-05728},
pages = {15021},
year = {2018},
abstract = {Flavin-binding fluorescent proteins (FPs) are genetically
encoded in vivo reporters, which are derived from microbial
and plant LOV photoreceptors. In this study, we
comparatively analyzed ROS formation and light-driven
antimicrobial efficacy of eleven LOV-based FPs. In
particular, we determined singlet oxygen (1O2) quantum
yields and superoxide photosensitization activities via
spectroscopic assays and performed cell toxicity experiments
in E. coli. Besides miniSOG and SOPP, which have been
engineered to generate 1O2, all of the other tested
flavoproteins were able to produce singlet oxygen and/or
hydrogen peroxide but exhibited remarkable differences in
ROS selectivity and yield. Accordingly, most LOV-FPs are
potent photosensitizers, which can be used for
light-controlled killing of bacteria. Furthermore, the two
variants Pp2FbFP and DsFbFP M49I, exhibiting preferential
photosensitization of singlet oxygen or singlet oxygen and
superoxide, respectively, were shown to be new tools for
studying specific ROS-induced cell signaling processes. The
tested LOV-FPs thus further expand the toolbox of
optogenetic sensitizers usable for a broad spectrum of
microbiological and biomedical applications.},
cin = {ICS-4 / IBG-1 / IMET},
ddc = {600},
cid = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)IBG-1-20101118 /
I:(DE-Juel1)IMET-20090612},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30301917},
UT = {WOS:000446802800027},
doi = {10.1038/s41598-018-33291-4},
url = {https://juser.fz-juelich.de/record/856064},
}