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@ARTICLE{Fabris:856429,
      author       = {Fabris, Gloria and Lucantonio, Alessandro and Hampe, Nico
                      and Noetzel, Erik and Hoffmann, Bernd and DeSimone, Antonio
                      and Merkel, Rudolf},
      title        = {{N}anoscale {T}opography and {P}oroelastic {P}roperties of
                      {M}odel {T}issue {B}reast {G}land {B}asement {M}embranes},
      journal      = {Biophysical journal},
      volume       = {115},
      number       = {9},
      issn         = {0006-3495},
      address      = {Bethesda, Md.},
      publisher    = {Soc.},
      reportid     = {FZJ-2018-05828},
      pages        = {1770-1782},
      year         = {2018},
      abstract     = {Basement membranes (BMs) are thin layers of condensed
                      extracellular matrix proteins serving as permeability
                      filters, cellular anchoring sites, and barriers against
                      cancer cell invasion. It is believed that their
                      biomechanical properties play a crucial role in determining
                      cellular behavior and response, especially in mechanically
                      active tissues like breast glands. Despite this, so far,
                      relatively little attention has been dedicated to their
                      analysis because of the difficulty of isolating and handling
                      such thin layers of material. Here, we isolated BMs derived
                      from MCF10A spheroids—three-dimensional breast gland model
                      systems mimicking in vitro the most relevant phenotypic
                      characteristics of human breast lobules—and characterized
                      them by atomic force microscopy, enhanced resolution
                      confocal microscopy, and scanning electron microscopy. By
                      performing atomic force microscopy height-clamp experiments,
                      we obtained force-relaxation curves that offered the first
                      biomechanical data on isolated breast gland BMs to our
                      knowledge. Based on enhanced resolution confocal microscopy
                      and scanning electron microscopy imaging data, we modeled
                      the system as a polymer network immersed in liquid and
                      described it as a poroelastic material. Finite-element
                      simulations matching the experimental force-relaxation
                      curves allowed for the first quantification, to our
                      knowledge, of the bulk and shear moduli of the membrane as
                      well as its water permeability. These results represent a
                      first step toward a deeper understanding of the mechanism of
                      tensional homeostasis regulating mammary gland activity as
                      well as its disruption during processes of membrane
                      breaching and metastatic invasion.},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552) / 553 - Physical
                      Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-552 / G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30322796},
      UT           = {WOS:000449422100018},
      doi          = {10.1016/j.bpj.2018.09.020},
      url          = {https://juser.fz-juelich.de/record/856429},
}