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@ARTICLE{Hfig:857784,
      author       = {Höfig, Henning and Cerminara, Michele and Ritter, Ilona
                      and Schöne, Antonie and Pohl, Martina and Steffen, Victoria
                      and Walter, Julia and Vergara Dal Pont, Ignacio and
                      Katranidis, Alexandros and Fitter, Jörg},
      title        = {{S}ingle-{M}olecule {S}tudies on a {FRET} {B}iosensor:
                      {L}essons from a {C}omparison of {F}luorescent {P}rotein
                      {E}quipped versus {D}ye-{L}abeled {S}pecies},
      journal      = {Molecules},
      volume       = {23},
      number       = {12},
      issn         = {1420-3049},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {FZJ-2018-06751},
      pages        = {3105 -},
      year         = {2018},
      abstract     = {Bacterial periplasmic binding proteins (PBPs) undergo a
                      pronounced ligand-induced conformational change which can be
                      employed to monitor ligand concentrations. The most common
                      strategy to take advantage of this conformational change for
                      a biosensor design is to use a Förster resonance energy
                      transfer (FRET) signal. This can be achieved by attaching
                      either two fluorescent proteins (FPs) or two organic
                      fluorescent dyes of different colors to the PBPs in order to
                      obtain an optical readout signal which is closely related to
                      the ligand concentration. In this study we compare a
                      FP-equipped and a dye-labeled version of the
                      glucose/galactose binding protein MglB at the
                      single-molecule level. The comparison demonstrates that
                      changes in the FRET signal upon glucose binding are more
                      pronounced for the FP-equipped sensor construct as compared
                      to the dye-labeled analog. Moreover, the FP-equipped sensor
                      showed a strong increase of the FRET signal under crowding
                      conditions whereas the dye-labeled sensor was not influenced
                      by crowding. The choice of a labeling scheme should
                      therefore be made depending on the application of a
                      FRET-based sensor.},
      cin          = {ICS-5 / IBT-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-5-20110106 / I:(DE-Juel1)VDB55},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30486450},
      UT           = {WOS:000454523000055},
      doi          = {10.3390/molecules23123105},
      url          = {https://juser.fz-juelich.de/record/857784},
}