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@ARTICLE{Martin:863860,
author = {Martin, Remy and Larsen, Andreas Haahr and Corey, Robin
Adam and Midtgaard, Søren Roi and Frielinghaus, Henrich and
Schaffitzel, Christiane and Arleth, Lise and Collinson, Ian},
title = {{S}tructure and {D}ynamics of the {C}entral {L}ipid {P}ool
and {P}roteins of the {B}acterial {H}olo-{T}ranslocon},
journal = {Biophysical journal},
volume = {116},
number = {10},
issn = {0006-3495},
address = {Bethesda, Md.},
publisher = {Soc.},
reportid = {FZJ-2019-03831},
pages = {1931 - 1940},
year = {2019},
abstract = {The bacterial Sec translocon, SecYEG, associates with
accessory proteins YidC and the SecDF-YajC subcom-plex to
form the bacterial holo-translocon (HTL). The HTL is a
dynamic and flexible protein transport machine capable of
coor-dinating protein secretion across the membrane and
efficient lateral insertion of nascent membrane proteins.
It has been hypothesized that a central lipid core
facilitates the controlled passage of membrane proteins into
the bilayer, ensuring the efficient formation of their
native state. By performing small-angle neutron scattering
on protein solubilized in ‘‘match-out’’ deuterated
detergent, we have been able to interrogate a
‘‘naked’’ HTL complex, with the scattering
contribution of the sur-rounding detergent micelle rendered
invisible. Such an approach has allowed the confirmation of
a lipid core within the HTL, which accommodates between 8
and 29 lipids. Coarse-grained molecular dynamics simulations
of the HTL also demon-strate a dynamic, central pool of
lipids. An opening at this lipid-rich region between YidC
and the SecY lateral gate may provide an exit gateway for
newly synthesized, correctly oriented, membrane protein
helices, or even small bundles of helices, to emerge from
the HTL.},
cin = {JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS1-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:31053257},
UT = {WOS:000468419300015},
doi = {10.1016/j.bpj.2019.04.002},
url = {https://juser.fz-juelich.de/record/863860},
}