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100 1 _ |a Martin, Remy
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245 _ _ |a Structure and Dynamics of the Central Lipid Pool and Proteins of the Bacterial Holo-Translocon
260 _ _ |a Bethesda, Md.
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520 _ _ |a The bacterial Sec translocon, SecYEG, associates with accessory proteins YidC and the SecDF-YajC subcom-plex to form the bacterial holo-translocon (HTL). The HTL is a dynamic and flexible protein transport machine capable of coor-dinating protein secretion across the membrane and efficient lateral insertion of nascent membrane proteins. It has been hypothesized that a central lipid core facilitates the controlled passage of membrane proteins into the bilayer, ensuring the efficient formation of their native state. By performing small-angle neutron scattering on protein solubilized in ‘‘match-out’’ deuterated detergent, we have been able to interrogate a ‘‘naked’’ HTL complex, with the scattering contribution of the sur-rounding detergent micelle rendered invisible. Such an approach has allowed the confirmation of a lipid core within the HTL, which accommodates between 8 and 29 lipids. Coarse-grained molecular dynamics simulations of the HTL also demon-strate a dynamic, central pool of lipids. An opening at this lipid-rich region between YidC and the SecY lateral gate may provide an exit gateway for newly synthesized, correctly oriented, membrane protein helices, or even small bundles of helices, to emerge from the HTL.
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700 1 _ |a Larsen, Andreas Haahr
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700 1 _ |a Corey, Robin Adam
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700 1 _ |a Midtgaard, Søren Roi
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700 1 _ |a Frielinghaus, Henrich
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700 1 _ |a Schaffitzel, Christiane
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700 1 _ |a Arleth, Lise
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700 1 _ |a Collinson, Ian
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773 _ _ |a 10.1016/j.bpj.2019.04.002
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