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@ARTICLE{Kater:864274,
      author       = {Kater, Lukas and Frieg, Benedikt and Berninghausen, Otto
                      and Gohlke, Holger and Beckmann, Roland and Kedrov, Alexej},
      title        = {{P}artially inserted nascent chain unzips the lateral gate
                      of the {S}ec translocon},
      journal      = {EMBO reports},
      volume       = {20},
      issn         = {1469-3178},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {FZJ-2019-04091},
      pages        = {e48191},
      year         = {2019},
      abstract     = {The Sec translocon provides the lipid bilayer entry for
                      ribosome‐bound nascent chains and thus facilitates
                      membrane protein biogenesis. Despite the appreciated role of
                      the native environment in the translocon:ribosome assembly,
                      structural information on the complex in the lipid membrane
                      is scarce. Here, we present a cryo‐electron
                      microscopy‐based structure of bacterial translocon SecYEG
                      in lipid nanodiscs and elucidate an early intermediate state
                      upon insertion of the FtsQ anchor domain. Insertion of the
                      short nascent chain causes initial displacements within the
                      lateral gate of the translocon, where α‐helices 2b, 7,
                      and 8 tilt within the membrane core to “unzip” the gate
                      at the cytoplasmic side. Molecular dynamics simulations
                      demonstrate that the conformational change is reversed in
                      the absence of the ribosome, and suggest that the accessory
                      α‐helices of SecE subunit modulate the lateral gate
                      conformation. Site‐specific cross‐linking validates that
                      the FtsQ nascent chain passes the lateral gate upon
                      insertion. The structure and the biochemical data suggest
                      that the partially inserted nascent chain remains highly
                      flexible until it acquires the transmembrane topology.},
      cin          = {ICS-6 / JSC / NIC},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106 / I:(DE-Juel1)JSC-20090406 /
                      I:(DE-Juel1)NIC-20090406},
      pnm          = {511 - Computational Science and Mathematical Methods
                      (POF3-511) / Forschergruppe Gohlke $(hkf7_20170501)$},
      pid          = {G:(DE-HGF)POF3-511 / $G:(DE-Juel1)hkf7_20170501$},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31379073},
      UT           = {WOS:000478845800001},
      doi          = {10.15252/embr.201948191},
      url          = {https://juser.fz-juelich.de/record/864274},
}