000864631 001__ 864631
000864631 005__ 20240619091955.0
000864631 0247_ $$2doi$$a10.1111/febs.15015
000864631 0247_ $$2ISSN$$a0014-2956
000864631 0247_ $$2ISSN$$a0945-5795
000864631 0247_ $$2ISSN$$a1432-1033
000864631 0247_ $$2ISSN$$a1742-464X
000864631 0247_ $$2ISSN$$a1742-4658
000864631 0247_ $$2Handle$$a2128/24258
000864631 0247_ $$2pmid$$apmid:31330084
000864631 0247_ $$2WOS$$aWOS:000479584400001
000864631 0247_ $$2altmetric$$aaltmetric:75524166
000864631 037__ $$aFZJ-2019-04336
000864631 082__ $$a610
000864631 1001_ $$0P:(DE-Juel1)168353$$aLorenz, Charlotte$$b0$$ufzj
000864631 245__ $$aFarnesylation of human guanylate‐binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization
000864631 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2020
000864631 3367_ $$2DRIVER$$aarticle
000864631 3367_ $$2DataCite$$aOutput Types/Journal article
000864631 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1580915111_7428
000864631 3367_ $$2BibTeX$$aARTICLE
000864631 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000864631 3367_ $$00$$2EndNote$$aJournal Article
000864631 520__ $$aHuman guanylate‐binding protein 1 (hGBP1) belongs to the family of dynamin‐like proteins and is activated by addition of nucleotides, leading to protein oligomerization and stimulated GTPase activity. In vivo, hGBP1 is post‐translationally modified by attachment of a farnesyl group yielding farn‐hGBP1. In this study, hydrodynamic differences in farn‐hGBP1 and unmodified hGBP1 were investigated using dynamic light scattering (DLS), analytical ultracentrifugation (AUC) and analytical size‐exclusion chromatography (SEC). In addition, we performed small‐angle X‐ray scattering (SAXS) experiments coupled with a SEC setup (SEC‐SAXS) to investigate structural properties of nonmodified hGBP1 and farn‐hGBP1 in solution. SEC‐SAXS measurements revealed that farnesylation keeps hGBP1 in its inactive monomeric and crystal‐like conformation in nucleotide‐free solution, whereas unmodified hGBP1 forms a monomer–dimer equilibrium both in the inactive ground state in nucleotide‐free solution as well as in the activated state that is trapped by addition of the nonhydrolysable GTP analogue GppNHp. Nonmodified hGBP1 is structurally perturbed as compared to farn‐hGBP. In particular, GppNHp binding leads to large structural rearrangements and higher conformational flexibility of the monomer and the dimer. Structural changes observed in the nonmodified protein are prerequisites for further oligomer assemblies of farn‐hGBP1 that occur in the presence of nucleotides.
000864631 536__ $$0G:(DE-HGF)POF3-552$$a552 - Engineering Cell Function (POF3-552)$$cPOF3-552$$fPOF III$$x0
000864631 588__ $$aDataset connected to CrossRef
000864631 7001_ $$0P:(DE-HGF)0$$aInce, Semra$$b1
000864631 7001_ $$0P:(DE-Juel1)174389$$aZhang, Tao$$b2
000864631 7001_ $$0P:(DE-Juel1)139572$$aCousin, Anneliese$$b3$$ufzj
000864631 7001_ $$0P:(DE-HGF)0$$aBatra‐Safferling, Renu$$b4
000864631 7001_ $$0P:(DE-HGF)0$$aNagel‐Steger, Luitgard$$b5
000864631 7001_ $$00000-0002-1824-3647$$aHerrmann, Christian$$b6
000864631 7001_ $$0P:(DE-Juel1)140278$$aStadler, Andreas M.$$b7$$eCorresponding author
000864631 773__ $$0PERI:(DE-600)2172518-4$$a10.1111/febs.15015$$gp. febs.15015$$n3$$p496-514$$tThe FEBS journal$$v287$$x1742-4658$$y2020
000864631 8564_ $$uhttps://juser.fz-juelich.de/record/864631/files/Lorenz_et_al-2020-The_FEBS_Journal.pdf$$yOpenAccess
000864631 8564_ $$uhttps://juser.fz-juelich.de/record/864631/files/Lorenz_et_al-2020-The_FEBS_Journal.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000864631 8767_ $$92019-07-20$$d2019-08-21$$eHybrid-OA$$jDEAL$$lDEAL: Wiley$$pFJ-18-1062.R2
000864631 909CO $$ooai:juser.fz-juelich.de:864631$$pdnbdelivery$$popenCost$$pVDB$$pdriver$$pOpenAPC_DEAL$$popen_access$$popenaire
000864631 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)168353$$aForschungszentrum Jülich$$b0$$kFZJ
000864631 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)139572$$aForschungszentrum Jülich$$b3$$kFZJ
000864631 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-HGF)0$$aForschungszentrum Jülich$$b4$$kFZJ
000864631 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)140278$$aForschungszentrum Jülich$$b7$$kFZJ
000864631 9131_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000864631 9141_ $$y2020
000864631 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000864631 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000864631 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000864631 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000864631 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFEBS J : 2017
000864631 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000864631 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000864631 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000864631 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000864631 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000864631 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000864631 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000864631 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000864631 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000864631 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List
000864631 9201_ $$0I:(DE-Juel1)JCNS-1-20110106$$kJCNS-1$$lNeutronenstreuung$$x0
000864631 9201_ $$0I:(DE-Juel1)ICS-1-20110106$$kICS-1$$lNeutronenstreuung$$x1
000864631 9201_ $$0I:(DE-Juel1)ICS-6-20110106$$kICS-6$$lStrukturbiochemie$$x2
000864631 9801_ $$aAPC
000864631 9801_ $$aFullTexts
000864631 980__ $$ajournal
000864631 980__ $$aVDB
000864631 980__ $$aUNRESTRICTED
000864631 980__ $$aI:(DE-Juel1)JCNS-1-20110106
000864631 980__ $$aI:(DE-Juel1)ICS-1-20110106
000864631 980__ $$aI:(DE-Juel1)ICS-6-20110106
000864631 980__ $$aAPC
000864631 981__ $$aI:(DE-Juel1)IBI-8-20200312
000864631 981__ $$aI:(DE-Juel1)JCNS-1-20110106
000864631 981__ $$aI:(DE-Juel1)IBI-7-20200312