TY  - JOUR
AU  - Belitsky, V.
AU  - Schütz, G. M.
TI  - RNA polymerase interactions and elongation rate
JO  - Journal of theoretical biology
VL  - 462
SN  - 0022-5193
CY  - Amsterdam
PB  - Elsevier Ltd.
M1  - FZJ-2019-04590
SP  - 370-380
PY  - 2019
AB  - We show that non-steric molecular interactions between RNA polymerase (RNAP) motors that move simultaneously on the same DNA track determine strongly the kinetics of transcription elongation. With a focus on the role of collisions and cooperation, we introduce a stochastic model that allows for the exact analytical computation of the stationary properties of transcription elongation as a function of RNAP density, their interaction strength, nucleoside triphosphate concentration, and rate of pyrophosphate release. Cooperative pushing, i.e., an enhancement of the average RNAP velocity and elongation rate, arises due to stochastic pushing which cannot be explained by steric hindrance alone. The cooperative effect requires a molecular repulsion in excess of a critical strength and disappears beyond a critical RNAP density, above which jamming due to collisions takes over. For strong repulsion and at the same time strong stochastic blocking, cooperative pushing at low RNAP densities is suppressed, but a reentrance regime at higher densities appears.
LB  - PUB:(DE-HGF)16
C6  - pmid:30496746
UR  - <Go to ISI:>//WOS:000455972600031
DO  - DOI:10.1016/j.jtbi.2018.11.025
UR  - https://juser.fz-juelich.de/record/865025
ER  -