Journal Article

FZJ-2019-04590

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png RNA polymerase interactions and elongation rate

Belitsky, V. ; Schütz, G. M. (Corresponding author)FZJ*

2019
Elsevier Ltd. Amsterdam

This record in other databases:    

Please use a persistent id in citations: http://hdl.handle.net/2128/22699  doi:10.1016/j.jtbi.2018.11.025

Abstract: We show that non-steric molecular interactions between RNA polymerase (RNAP) motors that move simultaneously on the same DNA track determine strongly the kinetics of transcription elongation. With a focus on the role of collisions and cooperation, we introduce a stochastic model that allows for the exact analytical computation of the stationary properties of transcription elongation as a function of RNAP density, their interaction strength, nucleoside triphosphate concentration, and rate of pyrophosphate release. Cooperative pushing, i.e., an enhancement of the average RNAP velocity and elongation rate, arises due to stochastic pushing which cannot be explained by steric hindrance alone. The cooperative effect requires a molecular repulsion in excess of a critical strength and disappears beyond a critical RNAP density, above which jamming due to collisions takes over. For strong repulsion and at the same time strong stochastic blocking, cooperative pushing at low RNAP densities is suppressed, but a reentrance regime at higher densities appears.

---

Contributing Institute(s):

1. Theorie der Weichen Materie und Biophysik (ICS-2)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2019

---

Database coverage:
; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---