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000865134 1001_ $$0P:(DE-Juel1)171954$$aSvetlova, Anastasia$$b0
000865134 245__ $$aComposite Lipid Bilayers from Cell Membrane Extracts and Artificial Mixes as a Cell Culture Platform
000865134 260__ $$aWashington, DC$$bACS Publ.$$c2019
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000865134 520__ $$aAn artificial lipid bilayer is the closest possible model for the cell membrane. Despite that, current methods of lipid bilayer assembly and functionalization do not provide a satisfactory mimic of the cell–cell contact due to the inability to recreate an asymmetrical multicomponent system. In the current work, a method to produce an integrated solid-supported lipid bilayer combining natural extracts from cell membranes and artificially made lipid vesicles is proposed. This simple method allows delivery of transmembrane proteins and components of the extracellular matrix into the substrate. Biocompatibility of the composite natural/artificial lipid bilayers is evaluated by their interactions with the cardiomyocyte-like HL-1 cell line. Compared with fully artificial mixes, composite lipid bilayers allow cells to adhere and develop a morphologically more normal cytoskeleton.
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000865134 7001_ $$0P:(DE-Juel1)176465$$aEllieroth, Jana$$b1
000865134 7001_ $$0P:(DE-Juel1)169481$$aMilos, Frano$$b2
000865134 7001_ $$0P:(DE-Juel1)128705$$aMaybeck, Vanessa$$b3
000865134 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, Andreas$$b4$$eCorresponding author
000865134 773__ $$0PERI:(DE-600)2005937-1$$a10.1021/acs.langmuir.9b00763$$gVol. 35, no. 24, p. 8076 - 8084$$n24$$p8076 - 8084$$tLangmuir$$v35$$x1520-5827$$y2019
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