Journal Article

FZJ-2019-04686

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Composite Lipid Bilayers from Cell Membrane Extracts and Artificial Mixes as a Cell Culture Platform

Svetlova, A.FZJ* ; Ellieroth, J.FZJ* ; Milos, F.FZJ* ; Maybeck, V.FZJ* ; Offenhäusser, A. (Corresponding author)FZJ*

2019
ACS Publ. Washington, DC

This record in other databases:      

Please use a persistent id in citations: doi:10.1021/acs.langmuir.9b00763

Abstract: An artificial lipid bilayer is the closest possible model for the cell membrane. Despite that, current methods of lipid bilayer assembly and functionalization do not provide a satisfactory mimic of the cell–cell contact due to the inability to recreate an asymmetrical multicomponent system. In the current work, a method to produce an integrated solid-supported lipid bilayer combining natural extracts from cell membranes and artificially made lipid vesicles is proposed. This simple method allows delivery of transmembrane proteins and components of the extracellular matrix into the substrate. Biocompatibility of the composite natural/artificial lipid bilayers is evaluated by their interactions with the cardiomyocyte-like HL-1 cell line. Compared with fully artificial mixes, composite lipid bilayers allow cells to adhere and develop a morphologically more normal cytoskeleton.

---

Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2019

---

Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

---