TY  - JOUR
AU  - Svetlova, Anastasia
AU  - Ellieroth, Jana
AU  - Milos, Frano
AU  - Maybeck, Vanessa
AU  - Offenhäusser, Andreas
TI  - Composite Lipid Bilayers from Cell Membrane Extracts and Artificial Mixes as a Cell Culture Platform
JO  - Langmuir
VL  - 35
IS  - 24
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2019-04686
SP  - 8076 - 8084
PY  - 2019
AB  - An artificial lipid bilayer is the closest possible model for the cell membrane. Despite that, current methods of lipid bilayer assembly and functionalization do not provide a satisfactory mimic of the cell–cell contact due to the inability to recreate an asymmetrical multicomponent system. In the current work, a method to produce an integrated solid-supported lipid bilayer combining natural extracts from cell membranes and artificially made lipid vesicles is proposed. This simple method allows delivery of transmembrane proteins and components of the extracellular matrix into the substrate. Biocompatibility of the composite natural/artificial lipid bilayers is evaluated by their interactions with the cardiomyocyte-like HL-1 cell line. Compared with fully artificial mixes, composite lipid bilayers allow cells to adhere and develop a morphologically more normal cytoskeleton.
LB  - PUB:(DE-HGF)16
C6  - pmid:31055920
UR  - <Go to ISI:>//WOS:000472682600054
DO  - DOI:10.1021/acs.langmuir.9b00763
UR  - https://juser.fz-juelich.de/record/865134
ER  -