%0 Journal Article
%A Höfig, Henning
%A Yukhnovets, Olessya
%A Remes, Cristina
%A Kempf, Noemie
%A Katranidis, Alexandros
%A Kempe, Daryan
%A Fitter, Jörg
%T Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
%J Communications biology
%V 2
%N 1
%@ 2399-3642
%C London
%I Springer Nature
%M FZJ-2019-05903
%P 459
%D 2019
%X Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:31840104
%U <Go to ISI:>//WOS:000502374300006
%R 10.1038/s42003-019-0709-7
%U https://juser.fz-juelich.de/record/866840