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@ARTICLE{Hfig:866840,
      author       = {Höfig, Henning and Yukhnovets, Olessya and Remes, Cristina
                      and Kempf, Noemie and Katranidis, Alexandros and Kempe,
                      Daryan and Fitter, Jörg},
      title        = {{B}rightness-gated two-color coincidence detection unravels
                      two distinct mechanisms in bacterial protein translation
                      initiation},
      journal      = {Communications biology},
      volume       = {2},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {FZJ-2019-05903},
      pages        = {459},
      year         = {2019},
      abstract     = {Life on the molecular scale is based on a complex interplay
                      of biomolecules under which the ability of binding is
                      crucial. Fluorescence based two-color coincidence detection
                      (TCCD) is commonly used to characterize molecular binding,
                      but suffers from an underestimation of coincident events.
                      Here, we introduce a brightness-gated TCCD which overcomes
                      this limitation and benchmark our approach with two
                      custom-made calibration samples. Applied to a cell-free
                      protein synthesis assay, brightness-gated TCCD unraveled a
                      previously disregarded mode of translation initiation in
                      bacteria.},
      cin          = {ICS-5 / ICS-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-5-20110106 / I:(DE-Juel1)ICS-2-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:31840104},
      UT           = {WOS:000502374300006},
      doi          = {10.1038/s42003-019-0709-7},
      url          = {https://juser.fz-juelich.de/record/866840},
}