Journal Article

FZJ-2019-06419

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization

Kolomytseva, M. P. (Corresponding author) ; Myasoedova, N. M. ; Chernykh, A. M. ; Gaidina, A. S. ; Shebanova, A. D. ; Baskunov, B. P. ; Aschenbrenner, J.FZJ* ; Rosengarten, J. F.FZJ* ; Renfeld, Z. V. ; Gasanov, N. B. ; Pinchuk, I. P. ; Classen, T.FZJ* ; Pietruszka, J.FZJ* ; Golovleva, L. A.

2019
Elsevier New York, NY [u.a.]

This record in other databases:      

Please use a persistent id in citations: doi:10.1016/j.ijbiomac.2019.07.056

Abstract: Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59, 65, and 61 kDa respectively) were more active in acidic conditions at around 70 °C. However, in contrast to laccases I and II, laccase III retained its activity (8–30%) and stability during at least one week of incubation at neutral conditions that allows its biotechnological application carried out at neutral environment. The activation phenomena for some of the purified laccases from L. strigosus 1566 during incubation at high temperature, different pH, and sulfates is shown and discussed. According to MALDI-TOF analysis, laccases I and II are most closely related to the laccase of Panus rudis (AAR13230). Transformation of phenylpropanoids by the predominant laccases of L. strigosus 1566 to different polymers was demonstrated, indicating a great potential for producing novel pharmaceutical valuable analogues of lignans, stilbenes, flavonoids, and etc.. The studied laccases, which are products of the same strain, can become a convenient model for further studies of the structural mechanisms of the shift of T-/pH-optima, activation, and T-/pH-stability.

---

Contributing Institute(s):

1. Institut für Bioorganische Chemie (HHUD) (IBOC)
2. Biotechnologie (IBG-1)

Research Program(s):

1. 581 - Biotechnology (POF3-581) (POF3-581)

Appears in the scientific report 2019

---

Database coverage:
; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

---