000868045 001__ 868045
000868045 005__ 20210130004045.0
000868045 037__ $$aFZJ-2019-06638
000868045 041__ $$aEnglish
000868045 1001_ $$0P:(DE-Juel1)179235$$aReinecke-Levi, Diana$$b0$$eCorresponding author$$ufzj
000868045 1112_ $$a21st EU BC&E$$cCopenhagen$$d2013-06-03 - 2013-06-07$$wDenmark
000868045 245__ $$aAutolysin extraction for improved protoplast preparation in the commercial microalga Haematococcus pluvialis (Chlorophyceae)
000868045 260__ $$c2013
000868045 29510 $$aProceedings of the 21st EU biomass conference and exhibition
000868045 300__ $$a001-002
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000868045 3367_ $$033$$2EndNote$$aConference Paper
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000868045 3367_ $$0PUB:(DE-HGF)8$$2PUB:(DE-HGF)$$aContribution to a conference proceedings$$bcontrib$$mcontrib$$s1579099007_7154
000868045 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$mcontb
000868045 520__ $$aHaematococcus pluvialis is a green freshwater microalga and the most important producer of the natural pigment astaxanthin. The red keto- carotenoid astaxanthin has become a commercially promising nutraceutical since in- vitro tests have shown it to have antioxidant capacities and immunostimulatory effects. Natural astaxanthin gains a high market value and an annual production volume of 200 Mill US$. However, until today molecular and biochemical tools to unravel some basic characteristics of this alga are still missing. One of the major drawbacks towards addressing this problem is the lack of reproducible methods of protoplast generation. Further, the astaxanthin extraction is rather difficult and cost intensive, due to a rigid cell wall formed in Haematococcus cysts. The formation of this strong cell wall is triggered by stress condition such as desiccation, nitrogen deficiency and high light stress. In this research, we focus on a novel method of protoplast preparation via endogenous cell wall- lytic enzymes (autolysin) generated by mature mother cells. Those autolytic enzymes are well studied and applied in Chlamydomonas reinhardtii. It is known that gametes and sporangia produce specific autolysins, which react stage and strain specific. In Haematococcus were the extraction and application of those endogenous cell wal llytic enzymes (autolysin) not yet described.
000868045 536__ $$0G:(DE-HGF)POF3-582$$a582 - Plant Science (POF3-582)$$cPOF3-582$$fPOF III$$x0
000868045 7001_ $$0P:(DE-HGF)0$$aBoussiba, Sammy$$b1
000868045 7001_ $$0P:(DE-HGF)0$$aZarka, Aliza$$b2
000868045 770__ $$z9788889407530
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000868045 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)179235$$aForschungszentrum Jülich$$b0$$kFZJ
000868045 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aBen-Gurion University $$b1
000868045 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$aBen-Gurion University $$b2
000868045 9131_ $$0G:(DE-HGF)POF3-582$$1G:(DE-HGF)POF3-580$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lKey Technologies for the Bioeconomy$$vPlant Science$$x0
000868045 920__ $$lno
000868045 9201_ $$0I:(DE-Juel1)IBG-2-20101118$$kIBG-2$$lPflanzenwissenschaften$$x0
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