guest ::
| Home > Publications database > Autolysin extraction for improved protoplast preparation in the commercial microalga Haematococcus pluvialis (Chlorophyceae) |
| Home > Publications database > Autolysin extraction for improved protoplast preparation in the commercial microalga Haematococcus pluvialis (Chlorophyceae) |
| Contribution to a conference proceedings/Contribution to a book | FZJ-2019-06638 |
; ;
2013
Abstract: Haematococcus pluvialis is a green freshwater microalga and the most important producer of the natural pigment astaxanthin. The red keto- carotenoid astaxanthin has become a commercially promising nutraceutical since in- vitro tests have shown it to have antioxidant capacities and immunostimulatory effects. Natural astaxanthin gains a high market value and an annual production volume of 200 Mill US$. However, until today molecular and biochemical tools to unravel some basic characteristics of this alga are still missing. One of the major drawbacks towards addressing this problem is the lack of reproducible methods of protoplast generation. Further, the astaxanthin extraction is rather difficult and cost intensive, due to a rigid cell wall formed in Haematococcus cysts. The formation of this strong cell wall is triggered by stress condition such as desiccation, nitrogen deficiency and high light stress. In this research, we focus on a novel method of protoplast preparation via endogenous cell wall- lytic enzymes (autolysin) generated by mature mother cells. Those autolytic enzymes are well studied and applied in Chlamydomonas reinhardtii. It is known that gametes and sporangia produce specific autolysins, which react stage and strain specific. In Haematococcus were the extraction and application of those endogenous cell wal llytic enzymes (autolysin) not yet described.
|
The record appears in these collections: |