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@INPROCEEDINGS{ReineckeLevi:868045,
      author       = {Reinecke-Levi, Diana and Boussiba, Sammy and Zarka, Aliza},
      title        = {{A}utolysin extraction for improved protoplast preparation
                      in the commercial microalga {H}aematococcus pluvialis
                      ({C}hlorophyceae)},
      reportid     = {FZJ-2019-06638},
      pages        = {001-002},
      year         = {2013},
      comment      = {Proceedings of the 21st EU biomass conference and
                      exhibition},
      booktitle     = {Proceedings of the 21st EU biomass
                       conference and exhibition},
      abstract     = {Haematococcus pluvialis is a green freshwater microalga and
                      the most important producer of the natural pigment
                      astaxanthin. The red keto- carotenoid astaxanthin has become
                      a commercially promising nutraceutical since in- vitro tests
                      have shown it to have antioxidant capacities and
                      immunostimulatory effects. Natural astaxanthin gains a high
                      market value and an annual production volume of 200 Mill
                      US$. However, until today molecular and biochemical tools to
                      unravel some basic characteristics of this alga are still
                      missing. One of the major drawbacks towards addressing this
                      problem is the lack of reproducible methods of protoplast
                      generation. Further, the astaxanthin extraction is rather
                      difficult and cost intensive, due to a rigid cell wall
                      formed in Haematococcus cysts. The formation of this strong
                      cell wall is triggered by stress condition such as
                      desiccation, nitrogen deficiency and high light stress. In
                      this research, we focus on a novel method of protoplast
                      preparation via endogenous cell wall- lytic enzymes
                      (autolysin) generated by mature mother cells. Those
                      autolytic enzymes are well studied and applied in
                      Chlamydomonas reinhardtii. It is known that gametes and
                      sporangia produce specific autolysins, which react stage and
                      strain specific. In Haematococcus were the extraction and
                      application of those endogenous cell wal llytic enzymes
                      (autolysin) not yet described.},
      month         = {Jun},
      date          = {2013-06-03},
      organization  = {21st EU $BC\&E,$ Copenhagen (Denmark),
                       3 Jun 2013 - 7 Jun 2013},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)8 / PUB:(DE-HGF)7},
      url          = {https://juser.fz-juelich.de/record/868045},
}