TY  - JOUR
AU  - Niether, Doreen
AU  - Sarter, Mona
AU  - Koenig, Bernd W.
AU  - Fitter, Jörg
AU  - Stadler, Andreas M.
AU  - Wiegand, Simone
TI  - Thermophoresis: The Case of Streptavidin and Biotin
JO  - Polymers
VL  - 12
IS  - 2
SN  - 2073-4360
CY  - Basel
PB  - MDPI
M1  - FZJ-2020-00655
SP  - 376 -
PY  - 2020
AB  - Thermophoretic behavior of a free protein changes upon ligand binding and gives access to information on the binding constants. The Soret effect has also been proven to be a promising tool to gain information on the hydration layer, as the temperature dependence of the thermodiffusion behavior is sensitive to solute–solvent interactions. In this work, we perform systematic thermophoretic measurements of the protein streptavidin (STV) and of the complex STV with biotin (B) using thermal diffusion forced Rayleigh scattering (TDFRS). Our experiments show that the temperature sensitivity of the Soret coefficient is reduced for the complex compared to the free protein. We discuss our data in comparison with recent quasi-elastic neutron scattering (QENS) measurements. As the QENS measurement has been performed in heavy water, we perform additional measurements in water/heavy water mixtures. Finally, we also elucidate the challenges arising from the quantiative thermophoretic study of complex multicomponent systems such as protein solutions.
LB  - PUB:(DE-HGF)16
C6  - pmid:32046223
UR  - <Go to ISI:>//WOS:000519849800126
DO  - DOI:10.3390/polym12020376
UR  - https://juser.fz-juelich.de/record/873342
ER  -