TY  - JOUR
AU  - Fan, Xiaoming
AU  - Hendriks, Johnny
AU  - Comini, Maddalena
AU  - Katranidis, Alexandros
AU  - Büldt, Georg
AU  - Gensch, Thomas
TI  - Spatial filter and its application in three-dimensional single molecule localization microscopy
JO  - Methods and applications in fluorescence
VL  - 8
IS  - 2
SN  - 2050-6120
CY  - Bristol
PB  - IOP Publ.
M1  - FZJ-2020-01007
SP  - 025008
PY  - 2020
AB  - Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to determine the axial positions of fluorescent molecules and nanoparticles on the nanometer scale by the usage of two spatial filters, which are placed in two otherwise identical emission detection channels. SFSMLM allows axial localization in a range of ca. 1.5 μm with a localization precision of 15 - 30 nm in axial direction. The technique was utilized for localizing and imaging small cellular structures – e.g. actin filaments, vesicles and mitochondria - in three dimensions.
LB  - PUB:(DE-HGF)16
C6  - pmid:32150730
UR  - <Go to ISI:>//WOS:000521503100001
DO  - DOI:10.1088/2050-6120/ab7e0f
UR  - https://juser.fz-juelich.de/record/873802
ER  -