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@ARTICLE{Fan:873802,
      author       = {Fan, Xiaoming and Hendriks, Johnny and Comini, Maddalena
                      and Katranidis, Alexandros and Büldt, Georg and Gensch,
                      Thomas},
      title        = {{S}patial filter and its application in three-dimensional
                      single molecule localization microscopy},
      journal      = {Methods and applications in fluorescence},
      volume       = {8},
      number       = {2},
      issn         = {2050-6120},
      address      = {Bristol},
      publisher    = {IOP Publ.},
      reportid     = {FZJ-2020-01007},
      pages        = {025008},
      year         = {2020},
      abstract     = {Single molecule localization microscopy (SMLM) allows the
                      imaging of cellular structures with resolutions five to ten
                      times below the diffraction limit of optical microscopy. It
                      was originally introduced as a two-dimensional technique
                      based on the localization of single emitters as projection
                      onto the x-y imaging plane. The determination of the axial
                      position of a fluorescent emitter is only possible by
                      additional information. Here we report a method (spatial
                      filter SMLM (SFSMLM)) that allows to determine the axial
                      positions of fluorescent molecules and nanoparticles on the
                      nanometer scale by the usage of two spatial filters, which
                      are placed in two otherwise identical emission detection
                      channels. SFSMLM allows axial localization in a range of ca.
                      1.5 μm with a localization precision of 15 - 30 nm in axial
                      direction. The technique was utilized for localizing and
                      imaging small cellular structures – e.g. actin filaments,
                      vesicles and mitochondria - in three dimensions.},
      cin          = {ICS-4 / ICS-5},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)ICS-5-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32150730},
      UT           = {WOS:000521503100001},
      doi          = {10.1088/2050-6120/ab7e0f},
      url          = {https://juser.fz-juelich.de/record/873802},
}