TY  - JOUR
AU  - Guo, Ting
AU  - Wu, Changtong
AU  - Offenhäusser, Andreas
AU  - Mayer, Dirk
TI  - A Novel Ratiometric Electrochemical Biosensor Based on a Split Aptamer for the Detection of Dopamine with Logic Gate Operations
JO  - Physica status solidi / A Applied research A
VL  - 217
IS  - 13
SN  - 1862-6319
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2020-01405
SP  - 1900924 -
PY  - 2020
AB  - A novel dual‐signal ratiometric electrochemical biosensor based on a split aptamer is developed. A common shortcoming of amperometric aptamer sensors is unspecific signaling due to ssDNA conformational flexibility. Herein, a ratiometric detection using two competitive redox‐labeled aptamer strands is proposed. The neurotransmitter dopamine (DA) is chosen as a model target due to its importance for signal processing in the central nervous system. A DA aptamer is split into two parts, S1 and S2, where strand S1 is tethered to the electrode, whereas the second strand is labeled with methylene blue (MB) and acts as a redox reporter. Another ssDNA strand (CS1) tagged with anthraquinone (AQ) is introduced, which is complementary to strand S1 and reports on surface‐tethered strands that remain in its virgin state. In the presence of DA, CS1 is released from the surface due to the formation of S1–S2–DA complexes, resulting in decreasing AQ and increasing MB Faraday currents. Furthermore, logic gate operations can be performed to either improve signal reliability or enlarge the detection range. This proof‐of‐concept study demonstrates that the splitting of a full aptamer into two parts together with the use of complementary ratiomeric tests can improve the reliability of the sensor response.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000552069000010
DO  - DOI:10.1002/pssa.201900924
UR  - https://juser.fz-juelich.de/record/874385
ER  -