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@ARTICLE{Guo:874385,
      author       = {Guo, Ting and Wu, Changtong and Offenhäusser, Andreas and
                      Mayer, Dirk},
      title        = {{A} {N}ovel {R}atiometric {E}lectrochemical {B}iosensor
                      {B}ased on a {S}plit {A}ptamer for the {D}etection of
                      {D}opamine with {L}ogic {G}ate {O}perations},
      journal      = {Physica status solidi / A Applied research A},
      volume       = {217},
      number       = {13},
      issn         = {1862-6319},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2020-01405},
      pages        = {1900924 -},
      year         = {2020},
      abstract     = {A novel dual‐signal ratiometric electrochemical biosensor
                      based on a split aptamer is developed. A common shortcoming
                      of amperometric aptamer sensors is unspecific signaling due
                      to ssDNA conformational flexibility. Herein, a ratiometric
                      detection using two competitive redox‐labeled aptamer
                      strands is proposed. The neurotransmitter dopamine (DA) is
                      chosen as a model target due to its importance for signal
                      processing in the central nervous system. A DA aptamer is
                      split into two parts, S1 and S2, where strand S1 is tethered
                      to the electrode, whereas the second strand is labeled with
                      methylene blue (MB) and acts as a redox reporter. Another
                      ssDNA strand (CS1) tagged with anthraquinone (AQ) is
                      introduced, which is complementary to strand S1 and reports
                      on surface‐tethered strands that remain in its virgin
                      state. In the presence of DA, CS1 is released from the
                      surface due to the formation of S1–S2–DA complexes,
                      resulting in decreasing AQ and increasing MB Faraday
                      currents. Furthermore, logic gate operations can be
                      performed to either improve signal reliability or enlarge
                      the detection range. This proof‐of‐concept study
                      demonstrates that the splitting of a full aptamer into two
                      parts together with the use of complementary ratiomeric
                      tests can improve the reliability of the sensor response.},
      cin          = {ICS-8},
      ddc          = {530},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {523 - Controlling Configuration-Based Phenomena (POF3-523)},
      pid          = {G:(DE-HGF)POF3-523},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000552069000010},
      doi          = {10.1002/pssa.201900924},
      url          = {https://juser.fz-juelich.de/record/874385},
}