000874906 001__ 874906 000874906 005__ 20220930130235.0 000874906 0247_ $$2doi$$a10.1107/S2059798320002995 000874906 0247_ $$2ISSN$$a0907-4449 000874906 0247_ $$2ISSN$$a1399-0047 000874906 0247_ $$2ISSN$$a2059-7983 000874906 0247_ $$2Handle$$a2128/24655 000874906 0247_ $$2altmetric$$aaltmetric:78265863 000874906 0247_ $$2pmid$$apmid:32254057 000874906 0247_ $$2WOS$$aWOS:000524555300004 000874906 037__ $$aFZJ-2020-01694 000874906 082__ $$a530 000874906 1001_ $$0P:(DE-Juel1)177870$$aBeckers, Maximilian$$b0$$eCorresponding author 000874906 245__ $$aConfidence maps: statistical inference of cryo-EM maps 000874906 260__ $$aOxford$$bWiley-Blackwell$$c2020 000874906 3367_ $$2DRIVER$$aarticle 000874906 3367_ $$2DataCite$$aOutput Types/Journal article 000874906 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1586177880_15239 000874906 3367_ $$2BibTeX$$aARTICLE 000874906 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000874906 3367_ $$00$$2EndNote$$aJournal Article 000874906 520__ $$aConfidence maps provide complementary information for interpreting cryo-EM densities as they indicate statistical significance with respect to background noise. They can be thresholded by specifying the expected false-discovery rate (FDR), and the displayed volume shows the parts of the map that have the corresponding level of significance. Here, the basic statistical concepts of confidence maps are reviewed and practical guidance is provided for their interpretation and usage inside the CCP-EM suite. Limitations of the approach are discussed and extensions towards other error criteria such as the family-wise error rate are presented. The observed map features can be rendered at a common isosurface threshold, which is particularly beneficial for the interpretation of weak and noisy densities. In the current article, a practical guide is provided to the recommended usage of confidence maps. 000874906 536__ $$0G:(DE-HGF)POF3-551$$a551 - Functional Macromolecules and Complexes (POF3-551)$$cPOF3-551$$fPOF III$$x0 000874906 588__ $$aDataset connected to CrossRef 000874906 7001_ $$00000-0002-4883-1546$$aPalmer, Colin M.$$b1 000874906 7001_ $$0P:(DE-Juel1)173949$$aSachse, Carsten$$b2$$eCorresponding author 000874906 773__ $$0PERI:(DE-600)2020492-9$$a10.1107/S2059798320002995$$gVol. 76, no. 4$$n4$$p1-8$$tActa crystallographica / D Biological crystallography online Section D$$v76$$x2059-7983$$y2020 000874906 8564_ $$uhttps://juser.fz-juelich.de/record/874906/files/rr5194.pdf$$yOpenAccess 000874906 8564_ $$uhttps://juser.fz-juelich.de/record/874906/files/rr5194.pdf?subformat=pdfa$$xpdfa$$yOpenAccess 000874906 8767_ $$d2021-01-28$$eHybrid-OA$$jDEAL$$lDEAL: Wiley 000874906 909CO $$ooai:juser.fz-juelich.de:874906$$pVDB$$pdriver$$pOpenAPC_DEAL$$popen_access$$popenaire$$popenCost$$pdnbdelivery 000874906 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)177870$$aForschungszentrum Jülich$$b0$$kFZJ 000874906 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)173949$$aForschungszentrum Jülich$$b2$$kFZJ 000874906 9131_ $$0G:(DE-HGF)POF3-551$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vFunctional Macromolecules and Complexes$$x0 000874906 9141_ $$y2020 000874906 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS 000874906 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences 000874906 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0 000874906 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search 000874906 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bACTA CRYSTALLOGR D : 2017 000874906 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection 000874906 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index 000874906 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded 000874906 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5 000874906 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess 000874906 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC 000874906 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database 000874906 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews 000874906 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline 000874906 915__ $$0StatID:(DE-HGF)0320$$2StatID$$aDBCoverage$$bPubMed Central 000874906 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz 000874906 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List 000874906 920__ $$lyes 000874906 9201_ $$0I:(DE-Juel1)ER-C-3-20170113$$kER-C-3$$lStrukturbiologie$$x0 000874906 9801_ $$aFullTexts 000874906 980__ $$ajournal 000874906 980__ $$aVDB 000874906 980__ $$aUNRESTRICTED 000874906 980__ $$aI:(DE-Juel1)ER-C-3-20170113 000874906 980__ $$aAPC