Journal Article

FZJ-2020-01694

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Confidence maps: statistical inference of cryo-EM maps

Beckers, M. (Corresponding author)FZJ* ; Palmer, C. M. ; Sachse, C. (Corresponding author)FZJ*

2020
Wiley-Blackwell Oxford

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Please use a persistent id in citations: http://hdl.handle.net/2128/24655  doi:10.1107/S2059798320002995

Abstract: Confidence maps provide complementary information for interpreting cryo-EM densities as they indicate statistical significance with respect to background noise. They can be thresholded by specifying the expected false-discovery rate (FDR), and the displayed volume shows the parts of the map that have the corresponding level of significance. Here, the basic statistical concepts of confidence maps are reviewed and practical guidance is provided for their interpretation and usage inside the CCP-EM suite. Limitations of the approach are discussed and extensions towards other error criteria such as the family-wise error rate are presented. The observed map features can be rendered at a common isosurface threshold, which is particularly beneficial for the interpretation of weak and noisy densities. In the current article, a practical guide is provided to the recommended usage of confidence maps.

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Contributing Institute(s):

1. Strukturbiologie (ER-C-3)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2020

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Database coverage:
; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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