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@ARTICLE{Stockhausen:875286,
      author       = {Stockhausen, Anne and Bürgers, Jana and Rodriguez-Gatica,
                      Juan Eduardo and Schweihoff, Jens and Merkel, Rudolf and
                      Prigge, Jens Markus and Schwarz, Martin Karl and
                      Kubitscheck, Ulrich},
      title        = {{H}ard-wired lattice light-sheet microscopy for imaging of
                      expanded samples},
      journal      = {Optics express},
      volume       = {28},
      number       = {10},
      issn         = {1094-4087},
      address      = {Washington, DC},
      publisher    = {Soc.},
      reportid     = {FZJ-2020-01920},
      pages        = {15587 -},
      year         = {2020},
      abstract     = {Light-sheet fluorescence microscopy (LSFM) helps
                      investigate small structures in developing cells and tissue
                      for three-dimensional localization microscopy and
                      large-field brain imaging in neuroscience. Lattice
                      light-sheet microscopy is a recent development with great
                      potential to improve axial resolution and usable field
                      sizes, thus improving imaging speed. In contrast to the
                      commonly employed Gaussian beams for light-sheet generation
                      in conventional LSFM, in lattice light-sheet microscopy an
                      array of low diverging Bessel beams with a suppressed side
                      lobe structure is used. We developed a facile elementary
                      lattice light-sheet microscope using a micro-fabricated
                      fixed ring mask for lattice light-sheet generation. In our
                      setup, optical hardware elements enable a stable and simple
                      illumination path without the need for spatial light
                      modulators. This setup, in combination with long-working
                      distance objectives and the possibility for simultaneous
                      dual-color imaging, provides optimal conditions for imaging
                      extended optically cleared tissue samples. We here present
                      experimental data of fluorescently stained neurons and
                      neurites from mouse hippocampus following tissue expansion
                      and demonstrate the high homogeneous resolution throughout
                      the entire imaged volume. Utilizing our purpose-built
                      lattice light-sheet microscope, we reached a homogeneous
                      excitation and an axial resolution of 1.2 µm over a field
                      of view of (333 µm)2.},
      cin          = {IBI-2},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBI-2-20200312},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32403583},
      UT           = {WOS:000538870000123},
      doi          = {10.1364/OE.393728},
      url          = {https://juser.fz-juelich.de/record/875286},
}