Journal Article

FZJ-2020-01920

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Hard-wired lattice light-sheet microscopy for imaging of expanded samples

Stockhausen, A. (Corresponding author) ; Bürgers, J. ; Rodriguez-Gatica, J. E. ; Schweihoff, J. ; Merkel, R.FZJ* ; Prigge, J. M.FZJ* ; Schwarz, M. K. ; Kubitscheck, U.

2020
Soc. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/24822  doi:10.1364/OE.393728

Abstract: Light-sheet fluorescence microscopy (LSFM) helps investigate small structures in developing cells and tissue for three-dimensional localization microscopy and large-field brain imaging in neuroscience. Lattice light-sheet microscopy is a recent development with great potential to improve axial resolution and usable field sizes, thus improving imaging speed. In contrast to the commonly employed Gaussian beams for light-sheet generation in conventional LSFM, in lattice light-sheet microscopy an array of low diverging Bessel beams with a suppressed side lobe structure is used. We developed a facile elementary lattice light-sheet microscope using a micro-fabricated fixed ring mask for lattice light-sheet generation. In our setup, optical hardware elements enable a stable and simple illumination path without the need for spatial light modulators. This setup, in combination with long-working distance objectives and the possibility for simultaneous dual-color imaging, provides optimal conditions for imaging extended optically cleared tissue samples. We here present experimental data of fluorescently stained neurons and neurites from mouse hippocampus following tissue expansion and demonstrate the high homogeneous resolution throughout the entire imaged volume. Utilizing our purpose-built lattice light-sheet microscope, we reached a homogeneous excitation and an axial resolution of 1.2 µm over a field of view of (333 µm)2.

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Contributing Institute(s):

1. Mechanobiologie (IBI-2)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2020

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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